Clinical Studies \ SESHA SKIN #002

OBJECTIVE: Comparison of epidermal cell proliferation produced by SESHA SKIN THERAPY A.C.E. Cream containing PET™ nutrient delivery system with Retin-A Cream (0.025%) and Oil of Olay Original Beauty Fluid.

PROTOCOL AND STUDY PARAMETERS: 7-day in-vivo study on five human subjects.

RESULTS: The A.C.E Cream significantly thickened the epidermis. The skin is plump and has characteristics of young, healthy skin.

Retin-A 0.025% produced a slight increase of skin cells, but the epidermis is damaged.

Oil of Olay did not show signs of cell proliferation.

CONCLUSION: Biopsies stained with hematoxylin and eosin at 400x magnification show that A.C.E. Cream is an effective stimulator of epidermal cells.

Thickness of Epidermis

Table 1. Epidermal Thickness in microns (micrometers) :

SUBJECT	A.C.E. CREAM	RETIN-A 0.025%	OIL OF OLAY
A B C D E	50.3 47.3 59.5 58.3 51.5	33 34 39 39 36	19.8 21 18 21 21
MEAN	53.4	36.2	20.2

Hematoxylin and Eosin Stain of sample skin biopsies @ 400x :

Hematoxylin and Eosin Stain of sample skin biopsies @ 400x